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The Clean Pathogen Kit is designed for high throughput and reliable isolation of high quality host genomic DNA, gram positive and negative bacterial DNA, fungal spore DNA, and viral DNA and viral RNA from tissue, urine, serum, and fecal samples. The kits protocol is fully scalable and due to the use of our magnetic bead purification technology, can besides manual usage, easily be automated once the samples have been lysed on liquid handling workstations (e.g. Hamilton STARTM, Thermo KingFisherTM Flex, Applied Biosystems® MagMAXTM, Qiagen BioSprint, and other liquid handling instruments). Take a look at the vast number of GC Biotech products we can provide for your research or business. ProcedureThe system combines the CleanNA technology with our specially formulated buffer system to eliminate the binding of PCR inhibiting compounds, present within the samples, onto our magnetic particles. Following grinding and lysis, the Nucleic Acids are bound to our CleanNA Particles surfaces. The CleanNA magnetic particles are separated from the lysates by using a magnetic separation device. Following a few rapid wash steps to remove trace contaminants, the purified DNA is eluted from the CleanNA particles for downstream applications using an elution buffer. Features and benefits
These features and more are available on the website. Take a look in our store and see which GC Biotech applications we can provide for your lab in terms of research or business tools. These activities can range from nucleic acid isolation, life sciences and DNA or RNA analysis. Different tasks ask for different tools, so we provide a wide range of products from respected suppliers. Want to learn more about the clean pathogen DNA kit? Visit the website and submit your contact info. There is more about the fields of research and ordering information. |
Veelgestelde vragen
Welke soorten monsters kunnen met de Clean Pathogen Kit worden verwerkt?▼
De kit is ontworpen voor isolatie uit weefsel, urine, serum en fecale monsters. Het kan host-DNA, bacterieel DNA (gram-positief en negatief), schimmelsporen-DNA en viraal DNA en RNA isoleren.
Kan de Clean Pathogen Kit worden geautomatiseerd?▼
Ja, de kit is volledig schaalbaar en kan eenvoudig worden geautomatiseerd op liquid handling workstations zoals Hamilton STAR, Thermo KingFisher Flex en Qiagen BioSprint na lysering van de monsters.
Wat is het voordeel van de CleanNA magnetische parels?▼
De CleanNA-technologie elimineert de binding van PCR-remmende stoffen aan de magnetische partikels, waardoor zuivere nucleïnezuren ontstaan die direct geschikt zijn voor vervolgonderzoeken.
Zijn er organische oplosmiddelen nodig bij deze isolatiemethode?▼
Nee, de Clean Pathogen Kit werkt zonder organische oplosmiddelen, wat het proces veiliger en schoner maakt voor laboratoriumgebruik.
Wat is inbegrepen in de Clean Pathogen Kit?▼
De kit bevat onder meer Clean Disruptor Plates voor optimale homogenisering van monsters, magnetische parels en alle benodigde buffers voor nucleïnezuurisolatie.
